Healing accelerator NOSF (Nano-OligoSaccharide Factor) is a compound with a chemical structure derived from the oligosaccharide family, known to have antiproteinase properties.
Combined with the other ingredients in the TLC layer, healing accelerator NOSF preferentially binds to the damaged zones, exerting its anti-metalloproteinase activity.
In the micro-environment of leg ulcers, pressure ulcers and diabetic foot ulcers and recurring wounds, TLC-NOSF corrects metabolic imbalances by neutralising excess proteases on the protein degradation of Extracellular Matrix components, thereby promoting the action of growth factors on the synthesis of new scar tissue (1).
Mode of action
1. Regulation of the action of Matrix Metalloproteinases
The efficacy of healing accelerator NOSF is optimised by the Soft-Adherent TLC layer, enabling it to preferentially bind to the damaged zones following gelling on contact with the wound exudate. As a result of the formation of this gel, the Soft-Adherent TLC-NOSF layer neutralises excess proteases, removing them from the wound bed and therefore restoring the balance of the wound by limiting their detrimental action.
2. Stimulation of dermal reconstruction
Regulation of the proteolytic activity against the ExtraCellular Matrix (ECM) components restores the metabolic balance within the wound and promotes the action of growth factors. Mobilisation / multiplication of dermal fibroblasts is possible again, accompanied by re-triggering of their activity in terms of synthesis of the new matrix ingredients required to start dermal granulation.
In vitro study
Proliferation of fibroblasts
Materials and methods
Normal human dermal fibroblasts (NHDF) are cultivated in DMEM (Dulbecco’s Modified Eagle’s Medium) spiked with 10% foetal calf serum. Pieces of dressing, cut to the size of the culture wells, are then applied to the cell layers for 24 and 48 hours. Cell proliferation is measured by incorporation of tritiated thymidine.
The UrgoStart® dressing significantly stimulates human fibroblast proliferation after 24h and 48h.
Effect on regulation of MMP activity and levels in the wound
Materials and methods
The overall activity of MMPs was measured in the normal human dermis fibroblast culture after incubation for 24 hours. The quantity of MMP1 was measured in macrophage cultures (human cell lines of U937 differentiated macrophages) by ELISA (Enzyme Linked ImmunoSorbent Assay).
|The Soft-Adherent TLC-NOSF dressing neutralises proteases activity.|
|The Soft-Adherent TLC-NOSF dressing reduces the level of MMP1 in the wound.|
The UrgoStart® dressing, which contains healing accelerator NOSF, neutralises overall proteases activity. It also induces a reduction in the level of MMP1 present in the culture medium. These activities on MMPs are equivalent to that of TLC-NOSFtechnology.
The action of Healing accelerator NOSF
(1)M. Bernard, Société BioAlternatives.
Effet in vitro d’un nouvel inhibiteur de métalloprotéases,
communication orale au Congrès des Plaies et Cicatrisations 2008, Paris